ssr_primer
Primer Details for TRINITY_DN80000_c2_g4_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTCATGAACCTCCTGCAT | GCTAGGCACAGGTGAATGGT | Start: 1503 |
| Tm | 60.107 | 60.035 | End: 1629 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | GCCTCATGAACCTCCTGCAT | TGCTAGGCACAGGTGAATGG | Start: 1503 |
| Tm | 60.107 | 60.035 | End: 1630 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
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| Sequence | GGTCTGCCCCAAGAATCCTC | AATACCAGGAAGTGCCGACG | Start: 393 |
| Tm | 60.107 | 60.108 | End: 507 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
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| Sequence | GCCTCATGAACCTCCTGCAT | GGAAGTCTCCTGCTAGGCAC | Start: 1503 |
| Tm | 60.107 | 59.823 | End: 1640 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
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| Sequence | GGTCTGCCCCAAGAATCCTC | AGCCTCACCGTTTCACCTTT | Start: 393 |
| Tm | 60.107 | 59.817 | End: 532 |
| Size | 20 | 20 | 140 |
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