ssr_primer
Primer Details for TRINITY_DN79995_c1_g1_i1 |
|---|
| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAAAAACTGCGCATGGTGA | AATGAGAGGCAGCTTGGCAT | Start: 647 |
| Tm | 59.968 | 60.033 | End: 771 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | AATGAGAGGCAGCTTGGCAT | TACCAAAAACTGCGCATGGC | Start: 2299 |
| Tm | 60.033 | 60.039 | End: 2426 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | CAGCACCTTCAATCCTCGGT | TCCGATCCAACGCCGTAAAA | Start: 1490 |
| Tm | 60.036 | 60.038 | End: 1639 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AAACACCTGTTGAAGCACGC | AATGAGAGGCAGCTTGGCAT | Start: 589 |
| Tm | 59.899 | 60.033 | End: 771 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
|---|
| Sequence | CTGTACGTTCCACCTGCGAT | TCACCATGCGCAGTTTTTGG | Start: 554 |
| Tm | 60.109 | 59.968 | End: 666 |
| Size | 20 | 20 | 113 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAAAAACTGCGCATGGTGA | AATGAGAGGCAGCTTGGCAT | Start: 647 |
| Tm | 59.968 | 60.033 | End: 771 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | AATGAGAGGCAGCTTGGCAT | TACCAAAAACTGCGCATGGC | Start: 2299 |
| Tm | 60.033 | 60.039 | End: 2426 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | CAGCACCTTCAATCCTCGGT | TCCGATCCAACGCCGTAAAA | Start: 1490 |
| Tm | 60.036 | 60.038 | End: 1639 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AAACACCTGTTGAAGCACGC | AATGAGAGGCAGCTTGGCAT | Start: 589 |
| Tm | 59.899 | 60.033 | End: 771 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
|---|
| Sequence | CTGTACGTTCCACCTGCGAT | TCACCATGCGCAGTTTTTGG | Start: 554 |
| Tm | 60.109 | 59.968 | End: 666 |
| Size | 20 | 20 | 113 |
(T)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAAAAACTGCGCATGGTGA | AATGAGAGGCAGCTTGGCAT | Start: 647 |
| Tm | 59.968 | 60.033 | End: 771 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | AATGAGAGGCAGCTTGGCAT | TACCAAAAACTGCGCATGGC | Start: 2299 |
| Tm | 60.033 | 60.039 | End: 2426 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | CAGCACCTTCAATCCTCGGT | TCCGATCCAACGCCGTAAAA | Start: 1490 |
| Tm | 60.036 | 60.038 | End: 1639 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AAACACCTGTTGAAGCACGC | AATGAGAGGCAGCTTGGCAT | Start: 589 |
| Tm | 59.899 | 60.033 | End: 771 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
|---|
| Sequence | CTGTACGTTCCACCTGCGAT | TCACCATGCGCAGTTTTTGG | Start: 554 |
| Tm | 60.109 | 59.968 | End: 666 |
| Size | 20 | 20 | 113 |
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