ssr_primer
Primer Details for TRINITY_DN79993_c0_g1_i5 |
|---|
| (T)30: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGTCGAAGTGGAACATT | CTCCAGAACGCATGACCACT | Start: 377 |
| Tm | 59.962 | 60.037 | End: 651 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | GCATGACCACTGCTTTCCAC | Start: 377 |
| Tm | 59.962 | 59.758 | End: 642 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | AGAACGCATGACCACTGCTT | Start: 377 |
| Tm | 59.962 | 60.25 | End: 647 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | TCCAGAACGCATGACCACTG | Start: 377 |
| Tm | 59.962 | 60.321 | End: 650 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
|---|
| Sequence | AGTGGTCATGCGTTCTGGAG | TGCGGCAAAAATAGACGTCG | Start: 632 |
| Tm | 60.037 | 59.559 | End: 797 |
| Size | 20 | 20 | 166 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGTCGAAGTGGAACATT | CTCCAGAACGCATGACCACT | Start: 377 |
| Tm | 59.962 | 60.037 | End: 651 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | GCATGACCACTGCTTTCCAC | Start: 377 |
| Tm | 59.962 | 59.758 | End: 642 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | AGAACGCATGACCACTGCTT | Start: 377 |
| Tm | 59.962 | 60.25 | End: 647 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | TCCAGAACGCATGACCACTG | Start: 377 |
| Tm | 59.962 | 60.321 | End: 650 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
|---|
| Sequence | AGTGGTCATGCGTTCTGGAG | TGCGGCAAAAATAGACGTCG | Start: 632 |
| Tm | 60.037 | 59.559 | End: 797 |
| Size | 20 | 20 | 166 |
(T)19(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGTCGAAGTGGAACATT | CTCCAGAACGCATGACCACT | Start: 377 |
| Tm | 59.962 | 60.037 | End: 651 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | GCATGACCACTGCTTTCCAC | Start: 377 |
| Tm | 59.962 | 59.758 | End: 642 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | AGAACGCATGACCACTGCTT | Start: 377 |
| Tm | 59.962 | 60.25 | End: 647 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | GGGGGTCGAAGTGGAACATT | TCCAGAACGCATGACCACTG | Start: 377 |
| Tm | 59.962 | 60.321 | End: 650 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
|---|
| Sequence | AGTGGTCATGCGTTCTGGAG | TGCGGCAAAAATAGACGTCG | Start: 632 |
| Tm | 60.037 | 59.559 | End: 797 |
| Size | 20 | 20 | 166 |
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