ssr_primer
Primer Details for TRINITY_DN79986_c6_g1_i1 |
|---|
| (A)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGCCGTAACATGACACC | GTGCCTGCATGAAGACTCCT | Start: 3003 |
| Tm | 60.037 | 60.036 | End: 3207 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | TCCAGCCGTAACATGACACC | ATCACTCGTGCCTGCATGAA | Start: 3003 |
| Tm | 60.037 | 60.036 | End: 3214 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | TCCAGCCGTAACATGACACC | AATCACTCGTGCCTGCATGA | Start: 3003 |
| Tm | 60.037 | 60.036 | End: 3215 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | TGCAATGTCTCAGGGGTGAC | AGGGCTACGCACACAATACC | Start: 2726 |
| Tm | 59.963 | 60.108 | End: 3002 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | TGCAATGTCTCAGGGGTGAC | TCTCCAAGACCTCCTCAGCA | Start: 2726 |
| Tm | 59.963 | 59.885 | End: 2939 |
| Size | 20 | 20 | 214 |
(C)13tcccaaacaactcccttcaatgcacctaaatacctcgagttacctaaatacctgcaactaaaatgaagcatgatcaatttctt(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGCCGTAACATGACACC | GTGCCTGCATGAAGACTCCT | Start: 3003 |
| Tm | 60.037 | 60.036 | End: 3207 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | TCCAGCCGTAACATGACACC | ATCACTCGTGCCTGCATGAA | Start: 3003 |
| Tm | 60.037 | 60.036 | End: 3214 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | TCCAGCCGTAACATGACACC | AATCACTCGTGCCTGCATGA | Start: 3003 |
| Tm | 60.037 | 60.036 | End: 3215 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | TGCAATGTCTCAGGGGTGAC | AGGGCTACGCACACAATACC | Start: 2726 |
| Tm | 59.963 | 60.108 | End: 3002 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | TGCAATGTCTCAGGGGTGAC | TCTCCAAGACCTCCTCAGCA | Start: 2726 |
| Tm | 59.963 | 59.885 | End: 2939 |
| Size | 20 | 20 | 214 |
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