ssr_primer
Primer Details for TRINITY_DN79928_c2_g1_i12 |
|---|
| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCTCCACTGCGGCAGAT | CGGTTCCTTTGTCTCTGGCT | Start: 1654 |
| Tm | 59.963 | 59.965 | End: 1860 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
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| Sequence | TCGCGCGTCTATGAATTCCA | TTGGCTGGCATACCGACAAT | Start: 348 |
| Tm | 59.898 | 60.035 | End: 556 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TCGCGCGTCTATGAATTCCA | ACCGACAATCCTTTCGCCAT | Start: 348 |
| Tm | 59.898 | 60.035 | End: 545 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
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| Sequence | TGGACAGTTCACCCTTGTCG | CGGTTCCTTTGTCTCTGGCT | Start: 1742 |
| Tm | 59.894 | 59.965 | End: 1860 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
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| Sequence | CCCTACCTCACGATGGATGC | ATCAGGGTTTACTGCTCGGC | Start: 1230 |
| Tm | 59.966 | 60.108 | End: 1484 |
| Size | 20 | 20 | 255 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCTCCACTGCGGCAGAT | CGGTTCCTTTGTCTCTGGCT | Start: 1654 |
| Tm | 59.963 | 59.965 | End: 1860 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | TCGCGCGTCTATGAATTCCA | TTGGCTGGCATACCGACAAT | Start: 348 |
| Tm | 59.898 | 60.035 | End: 556 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TCGCGCGTCTATGAATTCCA | ACCGACAATCCTTTCGCCAT | Start: 348 |
| Tm | 59.898 | 60.035 | End: 545 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | TGGACAGTTCACCCTTGTCG | CGGTTCCTTTGTCTCTGGCT | Start: 1742 |
| Tm | 59.894 | 59.965 | End: 1860 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | CCCTACCTCACGATGGATGC | ATCAGGGTTTACTGCTCGGC | Start: 1230 |
| Tm | 59.966 | 60.108 | End: 1484 |
| Size | 20 | 20 | 255 |
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