ssr_primer
Primer Details for TRINITY_DN79906_c0_g1_i9 |
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| (A)10gaaaaatattgttggcgctacggcaccgctgtataatagcaggagctgccaac(G)13ttagca(G)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATTGTACGCTCCGGATCG | CAATGGTAGAAGCGCGAACG | Start: 1466 |
| Tm | 59.97 | 59.975 | End: 1697 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
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| Sequence | CGTTCGCGCTTCTACCATTG | CATGGACCCGCATATCCCTC | Start: 1678 |
| Tm | 59.975 | 60.036 | End: 1833 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
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| Sequence | TCATGGGTCCATCCGGTACT | GATACGGGCAGACACCTACG | Start: 852 |
| Tm | 60.032 | 59.97 | End: 1030 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
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| Sequence | ATACAACAGTGGGAGCTGCC | CGATCCGGAGCGTACAATCA | Start: 1357 |
| Tm | 60.035 | 59.97 | End: 1485 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
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| Sequence | TGATTGTACGCTCCGGATCG | GCTCTACAGCCAAGCAGACA | Start: 1466 |
| Tm | 59.97 | 60.037 | End: 1650 |
| Size | 20 | 20 | 185 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATTGTACGCTCCGGATCG | CAATGGTAGAAGCGCGAACG | Start: 1466 |
| Tm | 59.97 | 59.975 | End: 1697 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CGTTCGCGCTTCTACCATTG | CATGGACCCGCATATCCCTC | Start: 1678 |
| Tm | 59.975 | 60.036 | End: 1833 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | TCATGGGTCCATCCGGTACT | GATACGGGCAGACACCTACG | Start: 852 |
| Tm | 60.032 | 59.97 | End: 1030 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | ATACAACAGTGGGAGCTGCC | CGATCCGGAGCGTACAATCA | Start: 1357 |
| Tm | 60.035 | 59.97 | End: 1485 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
|---|
| Sequence | TGATTGTACGCTCCGGATCG | GCTCTACAGCCAAGCAGACA | Start: 1466 |
| Tm | 59.97 | 60.037 | End: 1650 |
| Size | 20 | 20 | 185 |
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