ssr_primer
Primer Details for TRINITY_DN79865_c0_g1_i3 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAACCTTGCTCAGAAACG | GCGTTGTGCAATCTTGACGT | Start: 1034 |
| Tm | 60.04 | 60.041 | End: 1265 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CGCCAACCTTGCTCAGAAAC | GCGTTGTGCAATCTTGACGT | Start: 1033 |
| Tm | 60.04 | 60.041 | End: 1265 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | AAAACACGCCAACCTTGCTC | GCGTTGTGCAATCTTGACGT | Start: 1027 |
| Tm | 59.898 | 60.041 | End: 1265 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | CCAGGTTGTGGGCACTACTT | CTGTGTCCATAGTGCGTGGT | Start: 854 |
| Tm | 59.89 | 60.037 | End: 989 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CCAGGTTGTGGGCACTACTT | ACTGTGTCCATAGTGCGTGG | Start: 854 |
| Tm | 59.89 | 60.037 | End: 990 |
| Size | 20 | 20 | 137 |
(T)31: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAACCTTGCTCAGAAACG | GCGTTGTGCAATCTTGACGT | Start: 1034 |
| Tm | 60.04 | 60.041 | End: 1265 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CGCCAACCTTGCTCAGAAAC | GCGTTGTGCAATCTTGACGT | Start: 1033 |
| Tm | 60.04 | 60.041 | End: 1265 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | AAAACACGCCAACCTTGCTC | GCGTTGTGCAATCTTGACGT | Start: 1027 |
| Tm | 59.898 | 60.041 | End: 1265 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | CCAGGTTGTGGGCACTACTT | CTGTGTCCATAGTGCGTGGT | Start: 854 |
| Tm | 59.89 | 60.037 | End: 989 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CCAGGTTGTGGGCACTACTT | ACTGTGTCCATAGTGCGTGG | Start: 854 |
| Tm | 59.89 | 60.037 | End: 990 |
| Size | 20 | 20 | 137 |
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