ssr_primer
Primer Details for TRINITY_DN79853_c4_g4_i3 |
|---|
| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTACGCCTGTCTGTTCACC | GCTGAGAAAGAGGGGACACC | Start: 268 |
| Tm | 59.967 | 60.036 | End: 544 |
| Size | 20 | 20 | 277 |
| Primer 2 details |
|---|
| Sequence | TGTACGCCTGTCTGTTCACC | GTTGGACCGGTGTTCTGACT | Start: 268 |
| Tm | 59.967 | 59.894 | End: 416 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TGTACGCCTGTCTGTTCACC | AGTTGGACCGGTGTTCTGAC | Start: 268 |
| Tm | 59.967 | 59.894 | End: 417 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | GTCAGAACACCGGTCCAACT | GCTGAGAAAGAGGGGACACC | Start: 398 |
| Tm | 59.894 | 60.036 | End: 544 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | AGTCAGAACACCGGTCCAAC | GCTGAGAAAGAGGGGACACC | Start: 397 |
| Tm | 59.894 | 60.036 | End: 544 |
| Size | 20 | 20 | 148 |
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