ssr_primer
Primer Details for TRINITY_DN79750_c3_g3_i7 |
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| (T)13aaaatattaattatctttgcatttgcacggatcaggg(A)10c(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAAAGGACACGTACGGGTGC | GCAGAAACGAAAGCAGCCTC | Start: 3347 |
| Tm | 60.038 | 60.11 | End: 3567 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
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| Sequence | CACGTACGGGTGCACCTATT | GCAGAAACGAAAGCAGCCTC | Start: 3354 |
| Tm | 60.109 | 60.11 | End: 3567 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | AACTGTGGCACATGTTTGGC | TCTCCACAGTGGTCGGTACA | Start: 208 |
| Tm | 59.895 | 60.179 | End: 316 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | ACAGGAATGCAAGTGGGGTT | GCAGAAACGAAAGCAGCCTC | Start: 3377 |
| Tm | 59.812 | 60.11 | End: 3567 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | AACTGTGGCACATGTTTGGC | ATCTCTCCACAGTGGTCGGT | Start: 208 |
| Tm | 59.895 | 60.252 | End: 319 |
| Size | 20 | 20 | 112 |
(TAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAAAGGACACGTACGGGTGC | GCAGAAACGAAAGCAGCCTC | Start: 3347 |
| Tm | 60.038 | 60.11 | End: 3567 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | CACGTACGGGTGCACCTATT | GCAGAAACGAAAGCAGCCTC | Start: 3354 |
| Tm | 60.109 | 60.11 | End: 3567 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | AACTGTGGCACATGTTTGGC | TCTCCACAGTGGTCGGTACA | Start: 208 |
| Tm | 59.895 | 60.179 | End: 316 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | ACAGGAATGCAAGTGGGGTT | GCAGAAACGAAAGCAGCCTC | Start: 3377 |
| Tm | 59.812 | 60.11 | End: 3567 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | AACTGTGGCACATGTTTGGC | ATCTCTCCACAGTGGTCGGT | Start: 208 |
| Tm | 59.895 | 60.252 | End: 319 |
| Size | 20 | 20 | 112 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAAAGGACACGTACGGGTGC | GCAGAAACGAAAGCAGCCTC | Start: 3347 |
| Tm | 60.038 | 60.11 | End: 3567 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | CACGTACGGGTGCACCTATT | GCAGAAACGAAAGCAGCCTC | Start: 3354 |
| Tm | 60.109 | 60.11 | End: 3567 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | AACTGTGGCACATGTTTGGC | TCTCCACAGTGGTCGGTACA | Start: 208 |
| Tm | 59.895 | 60.179 | End: 316 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | ACAGGAATGCAAGTGGGGTT | GCAGAAACGAAAGCAGCCTC | Start: 3377 |
| Tm | 59.812 | 60.11 | End: 3567 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | AACTGTGGCACATGTTTGGC | ATCTCTCCACAGTGGTCGGT | Start: 208 |
| Tm | 59.895 | 60.252 | End: 319 |
| Size | 20 | 20 | 112 |
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