ssr_primer
Primer Details for TRINITY_DN79746_c5_g1_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGGGTGAAGACTCCTTGC | ATTCCAGGGACCGGAGATCA | Start: 493 |
| Tm | 59.965 | 60.031 | End: 686 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TTCGGGTGAAGACTCCTTGC | TGATTCCAGGGACCGGAGAT | Start: 493 |
| Tm | 59.965 | 60.031 | End: 688 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | TTCGGGTGAAGACTCCTTGC | ATGATTCCAGGGACCGGAGA | Start: 493 |
| Tm | 59.965 | 60.031 | End: 689 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
|---|
| Sequence | TCTCCGGTCCCTGGAATCAT | TGTCACCAGATGCCATGGAC | Start: 670 |
| Tm | 60.031 | 60.035 | End: 937 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | ATCTCCGGTCCCTGGAATCA | TGTCACCAGATGCCATGGAC | Start: 669 |
| Tm | 60.031 | 60.035 | End: 937 |
| Size | 20 | 20 | 269 |
(T)35: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGGGTGAAGACTCCTTGC | ATTCCAGGGACCGGAGATCA | Start: 493 |
| Tm | 59.965 | 60.031 | End: 686 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TTCGGGTGAAGACTCCTTGC | TGATTCCAGGGACCGGAGAT | Start: 493 |
| Tm | 59.965 | 60.031 | End: 688 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | TTCGGGTGAAGACTCCTTGC | ATGATTCCAGGGACCGGAGA | Start: 493 |
| Tm | 59.965 | 60.031 | End: 689 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
|---|
| Sequence | TCTCCGGTCCCTGGAATCAT | TGTCACCAGATGCCATGGAC | Start: 670 |
| Tm | 60.031 | 60.035 | End: 937 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | ATCTCCGGTCCCTGGAATCA | TGTCACCAGATGCCATGGAC | Start: 669 |
| Tm | 60.031 | 60.035 | End: 937 |
| Size | 20 | 20 | 269 |
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