ssr_primer
Primer Details for TRINITY_DN79742_c5_g1_i1 |
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| (G)34aggataatgcgtaacgagctaacaggaaaaatcagagagggtccatcagtcgaaaaaggctggtagtttctacctataccccataaat(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGAGCAAGCTTAAGGAAC | CGGGGCCCTACAGAAAAGTT | Start: 23 |
| Tm | 59.905 | 59.962 | End: 142 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
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| Sequence | AACTTTTCTGTAGGGCCCCG | CCGGTCTTGTTAAGGGCGAT | Start: 123 |
| Tm | 59.962 | 60.108 | End: 310 |
| Size | 20 | 20 | 188 |
| Primer 3 details |
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| Sequence | ACGTAGCTGAGATTCGCTGG | CCGGTCTTGTTAAGGGCGAT | Start: 60 |
| Tm | 59.899 | 60.108 | End: 310 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
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| Sequence | AACTTTTCTGTAGGGCCCCG | GCGTAAAGGGGGAAGAGGAG | Start: 123 |
| Tm | 59.962 | 59.822 | End: 275 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
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| Sequence | CGCGAGCAAGCTTAAGGAAC | GCGTAAAGGGGGAAGAGGAG | Start: 23 |
| Tm | 59.905 | 59.822 | End: 275 |
| Size | 20 | 20 | 253 |
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