ssr_primer
Primer Details for TRINITY_DN79734_c4_g3_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGAAGTGCGGAACCATGT | GGCTCATGTCAGGGGAGATG | Start: 539 |
| Tm | 59.891 | 59.892 | End: 789 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
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| Sequence | TAATCAGTCCGCGCAGTGAG | TAACTCGGCGTTGTCTCCAC | Start: 55 |
| Tm | 60.179 | 60.039 | End: 158 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
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| Sequence | ATTGCCCCATTTTGCCTTCG | TAACTCGGCGTTGTCTCCAC | Start: 0 |
| Tm | 59.75 | 60.039 | End: 158 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | GTGGAGACAACGCCGAGTTA | CTGAAGGCAAAGGCAAAGGC | Start: 139 |
| Tm | 60.039 | 60.32 | End: 402 |
| Size | 20 | 20 | 264 |
| Primer 5 details |
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| Sequence | GTGGAGACAACGCCGAGTTA | GCTGAAGGCAAAGGCAAAGG | Start: 139 |
| Tm | 60.039 | 60.32 | End: 403 |
| Size | 20 | 20 | 265 |
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