Primer Details for TRINITY_DN79654_c5_g5_i1 |
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| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGACTAAATGGATTTCACTTTGCA | AGGGCAGCATATATGTCATTGGT | Start: 124 |
| Tm | 59.225 | 59.925 | End: 225 |
| Size | 25 | 23 | 102 |
| Primer 2 details |
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| Sequence | GGACTAAATGGATTTCACTTTGCA | AGGGCAGCATATATGTCATTGGT | Start: 125 |
| Tm | 57.975 | 59.925 | End: 225 |
| Size | 24 | 23 | 101 |
| Primer 3 details |
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| Sequence | AGGACTAAATGGATTTCACTTTGCA | GGGCAGCATATATGTCATTGGT | Start: 124 |
| Tm | 59.225 | 58.575 | End: 224 |
| Size | 25 | 22 | 101 |
| Primer 4 details |
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| Sequence | AGGACTAAATGGATTTCACTTTGCAA | AGGGCAGCATATATGTCATTGGT | Start: 124 |
| Tm | 59.733 | 59.925 | End: 225 |
| Size | 26 | 23 | 102 |
| Primer 5 details |
|---|
| Sequence | AAGGACTAAATGGATTTCACTTTGCA | AGGGCAGCATATATGTCATTGGT | Start: 123 |
| Tm | 59.733 | 59.925 | End: 225 |
| Size | 26 | 23 | 103 |
