Primer Details for TRINITY_DN79630_c10_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGTTTAATTAAAAAGCGTTCACGT | CGAGGTCAATACTATCTCTTTAGGCA | Start: 126 |
| Tm | 59.032 | 60.016 | End: 225 |
| Size | 26 | 26 | 100 |
| Primer 2 details |
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| Sequence | ACTGTTTAATTAAAAAGCGTTCACGT | TCGAGGTCAATACTATCTCTTTAGGC | Start: 126 |
| Tm | 59.032 | 59.793 | End: 226 |
| Size | 26 | 26 | 101 |
| Primer 3 details |
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| Sequence | ACTGTTTAATTAAAAAGCGTTCACGTC | CGAGGTCAATACTATCTCTTTAGGCA | Start: 126 |
| Tm | 59.835 | 60.016 | End: 225 |
| Size | 27 | 26 | 100 |
| Primer 4 details |
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| Sequence | ACTGTTTAATTAAAAAGCGTTCACGT | CGAGGTCAATACTATCTCTTTAGGC | Start: 126 |
| Tm | 59.032 | 58.617 | End: 225 |
| Size | 26 | 25 | 100 |
| Primer 5 details |
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| Sequence | ACTGTTTAATTAAAAAGCGTTCACGTC | TCGAGGTCAATACTATCTCTTTAGGC | Start: 126 |
| Tm | 59.835 | 59.793 | End: 226 |
| Size | 27 | 26 | 101 |
