ssr_primer
Primer Details for TRINITY_DN79533_c5_g2_i1 |
|---|
| (A)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCCATTAGTTTCCCGGAC | TCGCTCGCATCGATGTTTCT | Start: 329 |
| Tm | 60.108 | 60.179 | End: 593 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | AGAAACATCGATGCGAGCGA | GTCCGGGAAACTAATGGCGA | Start: 198 |
| Tm | 60.179 | 60.108 | End: 348 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
|---|
| Sequence | CCGACTTCCCGGTATTACCC | GTCCGGGAAACTAATGGCGA | Start: 137 |
| Tm | 59.61 | 60.108 | End: 348 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
|---|
| Sequence | TCGCCATTAGTTTCCCGGAC | GAAAATGGAGAGGCGCCTTG | Start: 329 |
| Tm | 60.108 | 59.547 | End: 562 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | CAAGGCGCCTCTCCATTTTC | GTCCGGGAAACTAATGGCGA | Start: 167 |
| Tm | 59.547 | 60.108 | End: 348 |
| Size | 20 | 20 | 182 |
(A)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCCATTAGTTTCCCGGAC | TCGCTCGCATCGATGTTTCT | Start: 329 |
| Tm | 60.108 | 60.179 | End: 593 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | AGAAACATCGATGCGAGCGA | GTCCGGGAAACTAATGGCGA | Start: 198 |
| Tm | 60.179 | 60.108 | End: 348 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
|---|
| Sequence | CCGACTTCCCGGTATTACCC | GTCCGGGAAACTAATGGCGA | Start: 137 |
| Tm | 59.61 | 60.108 | End: 348 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
|---|
| Sequence | TCGCCATTAGTTTCCCGGAC | GAAAATGGAGAGGCGCCTTG | Start: 329 |
| Tm | 60.108 | 59.547 | End: 562 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | CAAGGCGCCTCTCCATTTTC | GTCCGGGAAACTAATGGCGA | Start: 167 |
| Tm | 59.547 | 60.108 | End: 348 |
| Size | 20 | 20 | 182 |
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