ssr_primer
Primer Details for TRINITY_DN79501_c3_g1_i1 |
|---|
| (G)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATCGAGCATGCCTCAAT | GCTTGCATGTGCTAGGAACG | Start: 56 |
| Tm | 59.818 | 59.902 | End: 175 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
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| Sequence | ACCATCGAGCATGCCTCAAT | CTAGGAACGAGCAGCCAGTC | Start: 56 |
| Tm | 59.818 | 60.179 | End: 164 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
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| Sequence | CGTTCCTAGCACATGCAAGC | GGGGACTTGAAAACGCAACG | Start: 156 |
| Tm | 59.902 | 60.318 | End: 282 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
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| Sequence | GAACCCCCTGTCAGCTGATC | GGGGACTTGAAAACGCAACG | Start: 107 |
| Tm | 60.107 | 60.318 | End: 282 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
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| Sequence | CGTTCCTAGCACATGCAAGC | GGGACTTGAAAACGCAACGT | Start: 156 |
| Tm | 59.902 | 59.621 | End: 281 |
| Size | 20 | 20 | 126 |
(A)16cattatcttggtaagttcaaaattagttcaacgttgcgttttcaagtccccaaatttcgggcaaagtttt(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATCGAGCATGCCTCAAT | GCTTGCATGTGCTAGGAACG | Start: 56 |
| Tm | 59.818 | 59.902 | End: 175 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | ACCATCGAGCATGCCTCAAT | CTAGGAACGAGCAGCCAGTC | Start: 56 |
| Tm | 59.818 | 60.179 | End: 164 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | CGTTCCTAGCACATGCAAGC | GGGGACTTGAAAACGCAACG | Start: 156 |
| Tm | 59.902 | 60.318 | End: 282 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GAACCCCCTGTCAGCTGATC | GGGGACTTGAAAACGCAACG | Start: 107 |
| Tm | 60.107 | 60.318 | End: 282 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CGTTCCTAGCACATGCAAGC | GGGACTTGAAAACGCAACGT | Start: 156 |
| Tm | 59.902 | 59.621 | End: 281 |
| Size | 20 | 20 | 126 |
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