ssr_primer
Primer Details for TRINITY_DN79487_c2_g1_i12 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAACCCCCGTTTTTCTCCCG | TCCCAGGGGAAAACCTCAGA | Start: 819 |
| Tm | 59.963 | 60.104 | End: 967 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | AGCCCAAGTAGCAGTGATCG | CGGGAGAAAAACGGGGGTTA | Start: 600 |
| Tm | 59.823 | 59.963 | End: 838 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | CCATACGGAACTCTGCGGTT | GCCAATCCGTCCCAAGATCA | Start: 298 |
| Tm | 60.108 | 60.107 | End: 428 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | CCATACGGAACTCTGCGGTT | TGCCAATCCGTCCCAAGATC | Start: 298 |
| Tm | 60.108 | 60.107 | End: 429 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | CCAAGTAGCAGTGATCGCGA | CGGGAGAAAAACGGGGGTTA | Start: 603 |
| Tm | 60.179 | 59.963 | End: 838 |
| Size | 20 | 20 | 236 |
(T)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAACCCCCGTTTTTCTCCCG | TCCCAGGGGAAAACCTCAGA | Start: 819 |
| Tm | 59.963 | 60.104 | End: 967 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | AGCCCAAGTAGCAGTGATCG | CGGGAGAAAAACGGGGGTTA | Start: 600 |
| Tm | 59.823 | 59.963 | End: 838 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | CCATACGGAACTCTGCGGTT | GCCAATCCGTCCCAAGATCA | Start: 298 |
| Tm | 60.108 | 60.107 | End: 428 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | CCATACGGAACTCTGCGGTT | TGCCAATCCGTCCCAAGATC | Start: 298 |
| Tm | 60.108 | 60.107 | End: 429 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | CCAAGTAGCAGTGATCGCGA | CGGGAGAAAAACGGGGGTTA | Start: 603 |
| Tm | 60.179 | 59.963 | End: 838 |
| Size | 20 | 20 | 236 |
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