ssr_primer
Primer Details for TRINITY_DN79436_c3_g5_i1 |
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| (T)16(TATTATTATTATTAAGAAAAAAATTGTACGACTGCCCCTTACGGGGCATTAATTACATTGTAGTTAATTTGTGCTTTACATTGTAGTTAATTTGTGCATTACATTTTAGTTAATTTGTGCATTACATTGTAGTTAATTTGTGCCGGTGTAGTTTATGGAAATAGTTACTTTATTTAGATTTAAATTATGTTTTGAGTAGAATTAGGCAAAAAAACCCCCCGGACGTTGCGGTCCGTTTTTCATACATTGATTTTCGGATTTTGCAAGAATATCGACGCCATATTGTACCCACTTACGTCACAGAGTAAATAAATCCTCTAAATGTTTTAAAAACGACCTAATAGTAAAAAGTCATTTGGACCTATTTCTGCCAAACGGAATTACTAGCATTTAGACAAGTGTCCTGGGACCCATAAGAAA)(TTA)4: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTACGACTGCCCCTTACG | ATGAAAAACGGACCGCAACG | Start: 57 |
| Tm | 59.756 | 60.041 | End: 276 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
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| Sequence | TTGTACGACTGCCCCTTACG | GAAAAACGGACCGCAACGTC | Start: 57 |
| Tm | 59.756 | 60.655 | End: 274 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
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| Sequence | CGTTGCGGTCCGTTTTTCAT | GGGTCCCAGGACACTTGTCT | Start: 257 |
| Tm | 60.041 | 61.128 | End: 445 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
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| Sequence | ATTGTACGACTGCCCCTTACG | ATGAAAAACGGACCGCAACG | Start: 56 |
| Tm | 60.135 | 60.041 | End: 276 |
| Size | 21 | 20 | 221 |
| Primer 5 details |
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| Sequence | GTACGACTGCCCCTTACGG | ATGAAAAACGGACCGCAACG | Start: 59 |
| Tm | 59.86 | 60.041 | End: 276 |
| Size | 19 | 20 | 218 |
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