ssr_primer
Primer Details for TRINITY_DN79407_c1_g1_i7 |
|---|
| (A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCGGTGGGAACAAAAAGC | ATCAATGAGTGGGTGCAGGG | Start: 391 |
| Tm | 59.968 | 60.034 | End: 578 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | CGGGGTGGAGTTTCTTCCTC | GCGGCAAGAAACTGCAAGAA | Start: 1706 |
| Tm | 60.036 | 59.969 | End: 1817 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | CCCTGCACCCACTCATTGAT | TCCACAGTGACGGGATGTTG | Start: 559 |
| Tm | 60.034 | 59.965 | End: 809 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | CAACATCCCGTCACTGTGGA | AGTCCAACTCCTTCTTGGCG | Start: 790 |
| Tm | 59.965 | 59.965 | End: 904 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | CAACATCCCGTCACTGTGGA | CGCCAAGTCCAACTCCTTCT | Start: 790 |
| Tm | 59.965 | 59.965 | End: 909 |
| Size | 20 | 20 | 120 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCGGTGGGAACAAAAAGC | ATCAATGAGTGGGTGCAGGG | Start: 391 |
| Tm | 59.968 | 60.034 | End: 578 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | CGGGGTGGAGTTTCTTCCTC | GCGGCAAGAAACTGCAAGAA | Start: 1706 |
| Tm | 60.036 | 59.969 | End: 1817 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | CCCTGCACCCACTCATTGAT | TCCACAGTGACGGGATGTTG | Start: 559 |
| Tm | 60.034 | 59.965 | End: 809 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | CAACATCCCGTCACTGTGGA | AGTCCAACTCCTTCTTGGCG | Start: 790 |
| Tm | 59.965 | 59.965 | End: 904 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | CAACATCCCGTCACTGTGGA | CGCCAAGTCCAACTCCTTCT | Start: 790 |
| Tm | 59.965 | 59.965 | End: 909 |
| Size | 20 | 20 | 120 |
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