ssr_primer
Primer Details for TRINITY_DN79360_c1_g2_i3 |
|---|
| (A)23t(A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTCTGAGTGAAGCTGTGG | GGCCTGGATCACACAGTTCA | Start: 724 |
| Tm | 59.967 | 59.963 | End: 860 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
|---|
| Sequence | ACGTCTGAGTGAAGCTGTGG | TGGCCTGGATCACACAGTTC | Start: 724 |
| Tm | 59.967 | 59.963 | End: 861 |
| Size | 20 | 20 | 138 |
| Primer 3 details |
|---|
| Sequence | ACCCATGCAAGTTATCGGCA | GATTGGAGTCAGCGACCCAA | Start: 1663 |
| Tm | 60.035 | 60.036 | End: 1838 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | ACGTCTGAGTGAAGCTGTGG | ATCTTCTTTGGCTGCTGGCT | Start: 724 |
| Tm | 59.967 | 59.961 | End: 991 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | GAACTGTGTGATCCAGGCCA | AACGGTTCCGTATCCCAACC | Start: 842 |
| Tm | 59.963 | 60.036 | End: 1011 |
| Size | 20 | 20 | 170 |
(A)27t(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTCTGAGTGAAGCTGTGG | GGCCTGGATCACACAGTTCA | Start: 724 |
| Tm | 59.967 | 59.963 | End: 860 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
|---|
| Sequence | ACGTCTGAGTGAAGCTGTGG | TGGCCTGGATCACACAGTTC | Start: 724 |
| Tm | 59.967 | 59.963 | End: 861 |
| Size | 20 | 20 | 138 |
| Primer 3 details |
|---|
| Sequence | ACCCATGCAAGTTATCGGCA | GATTGGAGTCAGCGACCCAA | Start: 1663 |
| Tm | 60.035 | 60.036 | End: 1838 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | ACGTCTGAGTGAAGCTGTGG | ATCTTCTTTGGCTGCTGGCT | Start: 724 |
| Tm | 59.967 | 59.961 | End: 991 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | GAACTGTGTGATCCAGGCCA | AACGGTTCCGTATCCCAACC | Start: 842 |
| Tm | 59.963 | 60.036 | End: 1011 |
| Size | 20 | 20 | 170 |
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