ssr_primer
Primer Details for TRINITY_DN79280_c0_g5_i1 |
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| (T)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCACCGGGATGATTTGCCA | ACTGCCGGAATGGTCTGATG | Start: 2035 |
| Tm | 59.961 | 60.108 | End: 2242 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
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| Sequence | ACCGGGATGATTTGCCACTT | ACTGCCGGAATGGTCTGATG | Start: 2038 |
| Tm | 59.96 | 60.108 | End: 2242 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
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| Sequence | TGCTGATGTCCGGGTTTTCA | TGGCAAATCATCCCGGTGAA | Start: 1779 |
| Tm | 59.89 | 59.961 | End: 2054 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
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| Sequence | TGCTGATGTCCGGGTTTTCA | AAGTGGCAAATCATCCCGGT | Start: 1779 |
| Tm | 59.89 | 59.96 | End: 2057 |
| Size | 20 | 20 | 279 |
| Primer 5 details |
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| Sequence | CCTCGACTGCCTCGGAATTT | GCCGAGTTGACATTCGAAGC | Start: 1169 |
| Tm | 60.108 | 59.905 | End: 1434 |
| Size | 20 | 20 | 266 |
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