ssr_primer
Primer Details for TRINITY_DN79236_c8_g1_i2 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACTGGTGCTGGCTACCAT | TCCTCCATACGTTCCCGCTA | Start: 626 |
| Tm | 60.035 | 60.107 | End: 896 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | GATCCCTTTTCCCGTGCGTA | GGCGATAGGGGAGGGAAATG | Start: 939 |
| Tm | 60.108 | 59.963 | End: 1057 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | TCCCTTTTCCCGTGCGTAAA | GGCGATAGGGGAGGGAAATG | Start: 941 |
| Tm | 59.892 | 59.963 | End: 1057 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | GAACTGGTGCTGGCTACCAT | TCTCTCCTGAGAAGTGCCCA | Start: 626 |
| Tm | 60.035 | 59.885 | End: 790 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GAACTGGTGCTGGCTACCAT | TTTTTCTTTGCGTGGCAGCA | Start: 626 |
| Tm | 60.035 | 59.826 | End: 868 |
| Size | 20 | 20 | 243 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACTGGTGCTGGCTACCAT | TCCTCCATACGTTCCCGCTA | Start: 626 |
| Tm | 60.035 | 60.107 | End: 896 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | GATCCCTTTTCCCGTGCGTA | GGCGATAGGGGAGGGAAATG | Start: 939 |
| Tm | 60.108 | 59.963 | End: 1057 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | TCCCTTTTCCCGTGCGTAAA | GGCGATAGGGGAGGGAAATG | Start: 941 |
| Tm | 59.892 | 59.963 | End: 1057 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | GAACTGGTGCTGGCTACCAT | TCTCTCCTGAGAAGTGCCCA | Start: 626 |
| Tm | 60.035 | 59.885 | End: 790 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GAACTGGTGCTGGCTACCAT | TTTTTCTTTGCGTGGCAGCA | Start: 626 |
| Tm | 60.035 | 59.826 | End: 868 |
| Size | 20 | 20 | 243 |
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