Primer Details for TRINITY_DN79199_c0_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTCGGGTGTGTATGCAGA | TCGTAAAACCACTGGAGATATTTTT | Start: 44 |
| Tm | 60.037 | 57.011 | End: 241 |
| Size | 20 | 25 | 198 |
| Primer 2 details |
|---|
| Sequence | TCAAAGAAATGCCAGAGGGTG | ACACTTAGTACTAGAATACACCGCA | Start: 24 |
| Tm | 58.482 | 59.351 | End: 127 |
| Size | 21 | 25 | 104 |
| Primer 3 details |
|---|
| Sequence | TCGGGTGTGTATGCAGAAGG | TCGTAAAACCACTGGAGATATTTTT | Start: 47 |
| Tm | 59.751 | 57.011 | End: 241 |
| Size | 20 | 25 | 195 |
| Primer 4 details |
|---|
| Sequence | TGGTTCGGGTGTGTATGCAG | TCGTAAAACCACTGGAGATATTTTT | Start: 43 |
| Tm | 60.321 | 57.011 | End: 241 |
| Size | 20 | 25 | 199 |
| Primer 5 details |
|---|
| Sequence | GTGGTTCGGGTGTGTATGCA | TCGTAAAACCACTGGAGATATTTTT | Start: 42 |
| Tm | 60.604 | 57.011 | End: 241 |
| Size | 20 | 25 | 200 |
