Primer Details for TRINITY_DN79194_c2_g1_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGATGGTTCTCAGAAAAATTCACA | CTGCCGTGCCAAGAAAGAAC | Start: 2 |
| Tm | 59.469 | 60.04 | End: 122 |
| Size | 25 | 20 | 121 |
| Primer 2 details |
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| Sequence | ACGATGGTTCTCAGAAAAATTCACA | TGCCAAGAAAGAACGCCGTA | Start: 2 |
| Tm | 59.469 | 60.25 | End: 116 |
| Size | 25 | 20 | 115 |
| Primer 3 details |
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| Sequence | TGACGATGGTTCTCAGAAAAATTCA | CTGCCGTGCCAAGAAAGAAC | Start: 0 |
| Tm | 59.237 | 60.04 | End: 122 |
| Size | 25 | 20 | 123 |
| Primer 4 details |
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| Sequence | TGACGATGGTTCTCAGAAAAATTCA | TGCCAAGAAAGAACGCCGTA | Start: 0 |
| Tm | 59.237 | 60.25 | End: 116 |
| Size | 25 | 20 | 117 |
| Primer 5 details |
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| Sequence | CGATGGTTCTCAGAAAAATTCACA | CTGCCGTGCCAAGAAAGAAC | Start: 3 |
| Tm | 57.999 | 60.04 | End: 122 |
| Size | 24 | 20 | 120 |
