ssr_primer
Primer Details for TRINITY_DN79179_c3_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGATAGGGCCTGTTGCAAA | AACCGGCATTTCAGAAGGGT | Start: 358 |
| Tm | 57.699 | 59.888 | End: 499 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
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| Sequence | AGGGCCTGTTGCAAATTACT | AACCGGCATTTCAGAAGGGT | Start: 363 |
| Tm | 57.691 | 59.888 | End: 499 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
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| Sequence | TGAGAGTGGAATCCGAGTTCA | AACCGGCATTTCAGAAGGGT | Start: 334 |
| Tm | 58.475 | 59.888 | End: 499 |
| Size | 21 | 20 | 166 |
| Primer 4 details |
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| Sequence | TGATAGGGCCTGTTGCAAAT | AACCGGCATTTCAGAAGGGT | Start: 359 |
| Tm | 57.47 | 59.888 | End: 499 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
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| Sequence | ATTGATAGGGCCTGTTGCAA | AACCGGCATTTCAGAAGGGT | Start: 357 |
| Tm | 57.47 | 59.888 | End: 499 |
| Size | 20 | 20 | 143 |
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