ssr_primer
Primer Details for TRINITY_DN79177_c5_g4_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCCTTCTTCCCCAGGATGA | TTTGTCATCGACGGAGCTCC | Start: 207 |
| Tm | 59.919 | 60.109 | End: 371 |
| Size | 21 | 20 | 165 |
| Primer 2 details |
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| Sequence | AGTCCTTCTTCCCCAGGATGA | CCAGCTATCCACCACAGTCC | Start: 207 |
| Tm | 59.919 | 59.821 | End: 353 |
| Size | 21 | 20 | 147 |
| Primer 3 details |
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| Sequence | AGTCCTTCTTCCCCAGGATGA | GCTCCAGCTATCCACCACAG | Start: 207 |
| Tm | 59.919 | 60.179 | End: 356 |
| Size | 21 | 20 | 150 |
| Primer 4 details |
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| Sequence | GGAGCTCCGTCGATGACAAA | TGCCTCTTTGATTTCTCCCGG | Start: 352 |
| Tm | 60.109 | 60.34 | End: 558 |
| Size | 20 | 21 | 207 |
| Primer 5 details |
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| Sequence | GGAGCTCCGTCGATGACAAA | GCCTCTTTGATTTCTCCCGGT | Start: 352 |
| Tm | 60.109 | 60.34 | End: 557 |
| Size | 20 | 21 | 206 |
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