ssr_primer
Primer Details for TRINITY_DN79162_c4_g4_i1 |
|---|
| (T)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTGGGCTTCACGAGACTC | GCGAGAAGATGGCCGTAAGA | Start: 26 |
| Tm | 59.966 | 59.898 | End: 151 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | TCTTACGGCCATCTTCTCGC | GTTCGGATGCTGGGACGTTA | Start: 132 |
| Tm | 59.898 | 60.109 | End: 340 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TGTTGGGCTTCACGAGACTC | TGCGAGAAGATGGCCGTAAG | Start: 26 |
| Tm | 59.966 | 60.179 | End: 152 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | TGTTGGGCTTCACGAGACTC | CTGCGAGAAGATGGCCGTAA | Start: 26 |
| Tm | 59.966 | 60.179 | End: 153 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
|---|
| Sequence | TTACGGCCATCTTCTCGCAG | GTTCGGATGCTGGGACGTTA | Start: 134 |
| Tm | 60.179 | 60.109 | End: 340 |
| Size | 20 | 20 | 207 |
(GCG)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTGGGCTTCACGAGACTC | GCGAGAAGATGGCCGTAAGA | Start: 26 |
| Tm | 59.966 | 59.898 | End: 151 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | TCTTACGGCCATCTTCTCGC | GTTCGGATGCTGGGACGTTA | Start: 132 |
| Tm | 59.898 | 60.109 | End: 340 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TGTTGGGCTTCACGAGACTC | TGCGAGAAGATGGCCGTAAG | Start: 26 |
| Tm | 59.966 | 60.179 | End: 152 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | TGTTGGGCTTCACGAGACTC | CTGCGAGAAGATGGCCGTAA | Start: 26 |
| Tm | 59.966 | 60.179 | End: 153 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
|---|
| Sequence | TTACGGCCATCTTCTCGCAG | GTTCGGATGCTGGGACGTTA | Start: 134 |
| Tm | 60.179 | 60.109 | End: 340 |
| Size | 20 | 20 | 207 |
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