ssr_primer
Primer Details for TRINITY_DN79139_c6_g2_i1 |
|---|
| (A)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGTGCGGTAACTCCACTT | TCAAAACTCCCCGACTCTGC | Start: 462 |
| Tm | 59.965 | 59.965 | End: 624 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | GCAGAGTCGGGGAGTTTTGA | TCCACGTCTAATGGGGGTCT | Start: 605 |
| Tm | 59.965 | 59.958 | End: 714 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | CCAGTGCGGTAACTCCACTT | TCCACGTCTAATGGGGGTCT | Start: 462 |
| Tm | 59.965 | 59.958 | End: 714 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
|---|
| Sequence | AGCCCGACTGATTCTCCTCT | GCCTCGCATCATGGCATTTT | Start: 4026 |
| Tm | 60.033 | 59.896 | End: 4197 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | AGCCCGACTGATTCTCCTCT | GAAGGGATTGACCTGGCCTC | Start: 4026 |
| Tm | 60.033 | 60.107 | End: 4212 |
| Size | 20 | 20 | 187 |
(G)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGTGCGGTAACTCCACTT | TCAAAACTCCCCGACTCTGC | Start: 462 |
| Tm | 59.965 | 59.965 | End: 624 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | GCAGAGTCGGGGAGTTTTGA | TCCACGTCTAATGGGGGTCT | Start: 605 |
| Tm | 59.965 | 59.958 | End: 714 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | CCAGTGCGGTAACTCCACTT | TCCACGTCTAATGGGGGTCT | Start: 462 |
| Tm | 59.965 | 59.958 | End: 714 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
|---|
| Sequence | AGCCCGACTGATTCTCCTCT | GCCTCGCATCATGGCATTTT | Start: 4026 |
| Tm | 60.033 | 59.896 | End: 4197 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | AGCCCGACTGATTCTCCTCT | GAAGGGATTGACCTGGCCTC | Start: 4026 |
| Tm | 60.033 | 60.107 | End: 4212 |
| Size | 20 | 20 | 187 |
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