ssr_primer
Primer Details for TRINITY_DN79128_c3_g3_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCCCGTCACTTGAAGGAA | ACTTGCGGAGATCCACCAAG | Start: 135 |
| Tm | 59.967 | 60.036 | End: 278 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
|---|
| Sequence | CGTCCCGTCACTTGAAGGAA | CTCACTTCCATCCTGCCGTT | Start: 135 |
| Tm | 59.967 | 60.036 | End: 380 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | TCATGGTCACAGCCACTGTC | TCAATATTACGCCGGCCTCC | Start: 161 |
| Tm | 59.965 | 59.966 | End: 422 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | TTTCCTTGGTGGATCTCCGC | TCAATATTACGCCGGCCTCC | Start: 255 |
| Tm | 60.035 | 59.966 | End: 422 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | TCATGGTCACAGCCACTGTC | AATATTACGCCGGCCTCCTG | Start: 161 |
| Tm | 59.965 | 59.965 | End: 420 |
| Size | 20 | 20 | 260 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTCCCGTCACTTGAAGGAA | ACTTGCGGAGATCCACCAAG | Start: 135 |
| Tm | 59.967 | 60.036 | End: 278 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
|---|
| Sequence | CGTCCCGTCACTTGAAGGAA | CTCACTTCCATCCTGCCGTT | Start: 135 |
| Tm | 59.967 | 60.036 | End: 380 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | TCATGGTCACAGCCACTGTC | TCAATATTACGCCGGCCTCC | Start: 161 |
| Tm | 59.965 | 59.966 | End: 422 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | TTTCCTTGGTGGATCTCCGC | TCAATATTACGCCGGCCTCC | Start: 255 |
| Tm | 60.035 | 59.966 | End: 422 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | TCATGGTCACAGCCACTGTC | AATATTACGCCGGCCTCCTG | Start: 161 |
| Tm | 59.965 | 59.965 | End: 420 |
| Size | 20 | 20 | 260 |
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