ssr_primer
Primer Details for TRINITY_DN79092_c2_g1_i9 |
|---|
| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATGGACGTTGATGTGGCA | TGCCGTTTGGTGTGTATCGA | Start: 1605 |
| Tm | 59.964 | 59.966 | End: 1788 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | GGCAGAAAGTTCCCCATCGA | GACTGGAGGGTGTACTTGGC | Start: 154 |
| Tm | 60.035 | 60.036 | End: 413 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | GCTTTTCTCCTCCGCTCCTT | TGCCACATCAACGTCCATGA | Start: 1412 |
| Tm | 60.036 | 59.964 | End: 1624 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | CCTCTGCAGCACAGCTACTT | GACTGGAGGGTGTACTTGGC | Start: 281 |
| Tm | 60.037 | 60.036 | End: 413 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | ACAGCTTTTCTCCTCCGCTC | TGCCACATCAACGTCCATGA | Start: 1409 |
| Tm | 60.037 | 59.964 | End: 1624 |
| Size | 20 | 20 | 216 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATGGACGTTGATGTGGCA | TGCCGTTTGGTGTGTATCGA | Start: 1605 |
| Tm | 59.964 | 59.966 | End: 1788 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | GGCAGAAAGTTCCCCATCGA | GACTGGAGGGTGTACTTGGC | Start: 154 |
| Tm | 60.035 | 60.036 | End: 413 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | GCTTTTCTCCTCCGCTCCTT | TGCCACATCAACGTCCATGA | Start: 1412 |
| Tm | 60.036 | 59.964 | End: 1624 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | CCTCTGCAGCACAGCTACTT | GACTGGAGGGTGTACTTGGC | Start: 281 |
| Tm | 60.037 | 60.036 | End: 413 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | ACAGCTTTTCTCCTCCGCTC | TGCCACATCAACGTCCATGA | Start: 1409 |
| Tm | 60.037 | 59.964 | End: 1624 |
| Size | 20 | 20 | 216 |
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