ssr_primer
Primer Details for TRINITY_DN79091_c3_g2_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGCTGAAGACTCCACAAA | TCGCACTCGCAGAACTTTCT | Start: 403 |
| Tm | 59.893 | 59.968 | End: 605 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | GCTGTGCATGAGACTCGACT | TCGCACTCGCAGAACTTTCT | Start: 491 |
| Tm | 60.109 | 59.968 | End: 605 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | TGCGCTTAATTGCCTGGAGA | TTTGTGGAGTCTTCAGCGCT | Start: 253 |
| Tm | 60.036 | 59.893 | End: 422 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | TGCGCTTAATTGCCTGGAGA | AGTCGAGTCTCATGCACAGC | Start: 253 |
| Tm | 60.036 | 60.109 | End: 510 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
|---|
| Sequence | ACTGTCATGATGTCCCGAGC | TCGCACTCGCAGAACTTTCT | Start: 386 |
| Tm | 59.823 | 59.968 | End: 605 |
| Size | 20 | 20 | 220 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGCTGAAGACTCCACAAA | TCGCACTCGCAGAACTTTCT | Start: 403 |
| Tm | 59.893 | 59.968 | End: 605 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | GCTGTGCATGAGACTCGACT | TCGCACTCGCAGAACTTTCT | Start: 491 |
| Tm | 60.109 | 59.968 | End: 605 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | TGCGCTTAATTGCCTGGAGA | TTTGTGGAGTCTTCAGCGCT | Start: 253 |
| Tm | 60.036 | 59.893 | End: 422 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | TGCGCTTAATTGCCTGGAGA | AGTCGAGTCTCATGCACAGC | Start: 253 |
| Tm | 60.036 | 60.109 | End: 510 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
|---|
| Sequence | ACTGTCATGATGTCCCGAGC | TCGCACTCGCAGAACTTTCT | Start: 386 |
| Tm | 59.823 | 59.968 | End: 605 |
| Size | 20 | 20 | 220 |
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