ssr_primer
Primer Details for TRINITY_DN79080_c4_g6_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATACAACCCAGCACCAGAGC | CTCCGACATCATAAGCCCCC | Start: 1697 |
| Tm | 60.035 | 59.965 | End: 1801 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
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| Sequence | AGATGACCCGCTTTGCTTCA | CTCCGACATCATAAGCCCCC | Start: 1639 |
| Tm | 59.963 | 59.965 | End: 1801 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
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| Sequence | ATACAACCCAGCACCAGAGC | AGTTGGACTCCACCTCTGGT | Start: 1697 |
| Tm | 60.035 | 60.105 | End: 1944 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
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| Sequence | GGGGGCTTATGATGTCGGAG | AGTTGGACTCCACCTCTGGT | Start: 1782 |
| Tm | 59.965 | 60.105 | End: 1944 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
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| Sequence | ACGACTTGAGCTTTGCCAGA | CCCCTTGTGGAAAAAGCTGC | Start: 1200 |
| Tm | 59.893 | 59.966 | End: 1321 |
| Size | 20 | 20 | 122 |
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