ssr_primer
Primer Details for TRINITY_DN79062_c3_g2_i6 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTACGGAGATTCGCGCTC | GCTGCCGCTTCAGTTGAAAA | Start: 435 |
| Tm | 59.971 | 59.969 | End: 545 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | TTTTCAACTGAAGCGGCAGC | ACAACGGTGCGGGAAAAATG | Start: 526 |
| Tm | 59.969 | 59.969 | End: 786 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TTTTCAACTGAAGCGGCAGC | AATCACCCAAGTCGCACGAT | Start: 526 |
| Tm | 59.969 | 60.036 | End: 747 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | TTTTCAACTGAAGCGGCAGC | GATCCACTCCTTGTTGCCGA | Start: 526 |
| Tm | 59.969 | 60.036 | End: 767 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | CATTTTTCCCGCACCGTTGT | CCACGTCGGGATGAGTTTGA | Start: 767 |
| Tm | 59.969 | 60.038 | End: 927 |
| Size | 20 | 20 | 161 |
(T)41: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTACGGAGATTCGCGCTC | GCTGCCGCTTCAGTTGAAAA | Start: 435 |
| Tm | 59.971 | 59.969 | End: 545 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | TTTTCAACTGAAGCGGCAGC | ACAACGGTGCGGGAAAAATG | Start: 526 |
| Tm | 59.969 | 59.969 | End: 786 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TTTTCAACTGAAGCGGCAGC | AATCACCCAAGTCGCACGAT | Start: 526 |
| Tm | 59.969 | 60.036 | End: 747 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | TTTTCAACTGAAGCGGCAGC | GATCCACTCCTTGTTGCCGA | Start: 526 |
| Tm | 59.969 | 60.036 | End: 767 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | CATTTTTCCCGCACCGTTGT | CCACGTCGGGATGAGTTTGA | Start: 767 |
| Tm | 59.969 | 60.038 | End: 927 |
| Size | 20 | 20 | 161 |
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