ssr_primer
Primer Details for TRINITY_DN79045_c3_g8_i1 |
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| (G)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGGGGAGGTAGCACAAAA | CTGCAACAGGCCCATTGGTC | Start: 42 |
| Tm | 59.888 | 61.881 | End: 270 |
| Size | 20 | 20 | 229 |
| Primer 2 details |
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| Sequence | GGTGGGGAGGTAGCACAAAA | TGCAACAGGCCCATTGGTC | Start: 42 |
| Tm | 59.888 | 60.908 | End: 269 |
| Size | 20 | 19 | 228 |
| Primer 3 details |
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| Sequence | GGTGGGGAGGTAGCACAAAA | CTGCAACAGGCCCATTGGT | Start: 42 |
| Tm | 59.888 | 60.91 | End: 270 |
| Size | 20 | 19 | 229 |
| Primer 4 details |
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| Sequence | AATTTTGCAAGCCCCGTCTG | CTGCAACAGGCCCATTGGTC | Start: 96 |
| Tm | 59.682 | 61.881 | End: 270 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
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| Sequence | CACTGCCAGACTGCCAGATT | CTGCAACAGGCCCATTGGTC | Start: 128 |
| Tm | 60.322 | 61.881 | End: 270 |
| Size | 20 | 20 | 143 |
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