ssr_primer
Primer Details for TRINITY_DN79007_c3_g1_i1 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGGGTCTGTGAAGAGCTT | TTCCTCACTTTTCCCGGTCG | Start: 824 |
| Tm | 59.964 | 59.966 | End: 932 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | AAAACCAGACCCACCGACTC | TTCCTCACTTTTCCCGGTCG | Start: 656 |
| Tm | 59.892 | 59.966 | End: 932 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | AAAACCAGACCCACCGACTC | AAGCTCTTCACAGACCCTGC | Start: 656 |
| Tm | 59.892 | 59.964 | End: 843 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | ACTGAAGCGTGTCGAAGAGG | GAGTCGGTGGGTCTGGTTTT | Start: 444 |
| Tm | 60.039 | 59.892 | End: 675 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | TCTTCGATTGACCCGTTCCG | CCTCTTCGACACGCTTCAGT | Start: 199 |
| Tm | 60.109 | 60.039 | End: 463 |
| Size | 20 | 20 | 265 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGGGTCTGTGAAGAGCTT | TTCCTCACTTTTCCCGGTCG | Start: 824 |
| Tm | 59.964 | 59.966 | End: 932 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | AAAACCAGACCCACCGACTC | TTCCTCACTTTTCCCGGTCG | Start: 656 |
| Tm | 59.892 | 59.966 | End: 932 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | AAAACCAGACCCACCGACTC | AAGCTCTTCACAGACCCTGC | Start: 656 |
| Tm | 59.892 | 59.964 | End: 843 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | ACTGAAGCGTGTCGAAGAGG | GAGTCGGTGGGTCTGGTTTT | Start: 444 |
| Tm | 60.039 | 59.892 | End: 675 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | TCTTCGATTGACCCGTTCCG | CCTCTTCGACACGCTTCAGT | Start: 199 |
| Tm | 60.109 | 60.039 | End: 463 |
| Size | 20 | 20 | 265 |
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