Primer Details for TRINITY_DN78986_c1_g4_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCATTCATGAACCAAACAATCCT | TTTGTGACAGAAATTTCTCAAGAGT | Start: 168 |
| Tm | 58.914 | 57.37 | End: 283 |
| Size | 24 | 25 | 116 |
| Primer 2 details |
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| Sequence | TCCATTCATGAACCAAACAATCCT | TGTGACAGAAATTTCTCAAGAGTAGA | Start: 168 |
| Tm | 58.914 | 58.274 | End: 281 |
| Size | 24 | 26 | 114 |
| Primer 3 details |
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| Sequence | TCCATTCATGAACCAAACAATCC | TTTGTGACAGAAATTTCTCAAGAGT | Start: 168 |
| Tm | 57.596 | 57.37 | End: 283 |
| Size | 23 | 25 | 116 |
| Primer 4 details |
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| Sequence | TCCATTCATGAACCAAACAATCCT | TGTGACAGAAATTTCTCAAGAGTAG | Start: 168 |
| Tm | 58.914 | 57.047 | End: 281 |
| Size | 24 | 25 | 114 |
| Primer 5 details |
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| Sequence | CCATTCATGAACCAAACAATCCT | TTTGTGACAGAAATTTCTCAAGAGT | Start: 169 |
| Tm | 57.59 | 57.37 | End: 283 |
| Size | 23 | 25 | 115 |
