ssr_primer
Primer Details for TRINITY_DN78977_c0_g1_i10 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAAGAAACTGCCGAGGAC | AGATTTCTGGCCTCACCTGC | Start: 846 |
| Tm | 59.965 | 60.035 | End: 1041 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | TGCAGCAGCTCATGTCAGAA | GTCCTCGGCAGTTTCTTGGA | Start: 699 |
| Tm | 59.964 | 59.965 | End: 865 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
|---|
| Sequence | TCAAGGCTTAGCGGAGTGTG | TTCTGACATGAGCTGCTGCA | Start: 442 |
| Tm | 60.037 | 59.964 | End: 718 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
|---|
| Sequence | GCCCCTGAGTGGTTTGATGA | CACACTCCGCTAAGCCTTGA | Start: 311 |
| Tm | 59.962 | 60.037 | End: 461 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | AGCCCCTGAGTGGTTTGATG | CACACTCCGCTAAGCCTTGA | Start: 310 |
| Tm | 59.961 | 60.037 | End: 461 |
| Size | 20 | 20 | 152 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAAGAAACTGCCGAGGAC | AGATTTCTGGCCTCACCTGC | Start: 846 |
| Tm | 59.965 | 60.035 | End: 1041 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | TGCAGCAGCTCATGTCAGAA | GTCCTCGGCAGTTTCTTGGA | Start: 699 |
| Tm | 59.964 | 59.965 | End: 865 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
|---|
| Sequence | TCAAGGCTTAGCGGAGTGTG | TTCTGACATGAGCTGCTGCA | Start: 442 |
| Tm | 60.037 | 59.964 | End: 718 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
|---|
| Sequence | GCCCCTGAGTGGTTTGATGA | CACACTCCGCTAAGCCTTGA | Start: 311 |
| Tm | 59.962 | 60.037 | End: 461 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | AGCCCCTGAGTGGTTTGATG | CACACTCCGCTAAGCCTTGA | Start: 310 |
| Tm | 59.961 | 60.037 | End: 461 |
| Size | 20 | 20 | 152 |
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