ssr_primer
Primer Details for TRINITY_DN78962_c3_g4_i2 |
|---|
| (A)10t(A)27: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGATCACACCCCCAACAG | CTCAACAGACAACCGACCGA | Start: 389 |
| Tm | 60.035 | 59.968 | End: 568 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | GCGGTAGAGTTGAGTGGGAG | CTCAACAGACAACCGACCGA | Start: 462 |
| Tm | 59.826 | 59.968 | End: 568 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | TCGGTCGGTTGTCTGTTGAG | CCGCTAGAAGGCAGGCTTTA | Start: 549 |
| Tm | 59.968 | 59.822 | End: 677 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
|---|
| Sequence | TCGGTCGGTTGTCTGTTGAG | ATTTTAGCAGCGCCGCTAGA | Start: 549 |
| Tm | 59.968 | 60.179 | End: 689 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | GGAGATCACACCCCCAACAG | CCCCACACCCTGACCATTTT | Start: 389 |
| Tm | 60.035 | 60.179 | End: 516 |
| Size | 20 | 20 | 128 |
(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGATCACACCCCCAACAG | CTCAACAGACAACCGACCGA | Start: 389 |
| Tm | 60.035 | 59.968 | End: 568 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | GCGGTAGAGTTGAGTGGGAG | CTCAACAGACAACCGACCGA | Start: 462 |
| Tm | 59.826 | 59.968 | End: 568 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | TCGGTCGGTTGTCTGTTGAG | CCGCTAGAAGGCAGGCTTTA | Start: 549 |
| Tm | 59.968 | 59.822 | End: 677 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
|---|
| Sequence | TCGGTCGGTTGTCTGTTGAG | ATTTTAGCAGCGCCGCTAGA | Start: 549 |
| Tm | 59.968 | 60.179 | End: 689 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | GGAGATCACACCCCCAACAG | CCCCACACCCTGACCATTTT | Start: 389 |
| Tm | 60.035 | 60.179 | End: 516 |
| Size | 20 | 20 | 128 |
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