ssr_primer
Primer Details for TRINITY_DN78869_c1_g1_i3 |
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| (T)32: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTGGGATTGGAAGGCAAG | TATTGGTCTTCCGGGGTTGC | Start: 699 |
| Tm | 59.96 | 60.035 | End: 949 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
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| Sequence | CAAAACTTCATCGCCGCACA | TTGCGTCTTTGATCCAGGCT | Start: 1783 |
| Tm | 60.041 | 59.963 | End: 1948 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
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| Sequence | TGTCTGAGAAACACGCTGCT | GAGCTCGCAAATCTTCGCTG | Start: 1119 |
| Tm | 59.895 | 59.974 | End: 1334 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
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| Sequence | GCAACCCCGGAAGACCAATA | AGCAGCGTGTTTCTCAGACA | Start: 930 |
| Tm | 60.035 | 59.895 | End: 1138 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
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| Sequence | AGCCTGGATCAAAGACGCAA | TCCTCCTGCGGCACATATTC | Start: 1929 |
| Tm | 59.963 | 59.893 | End: 2123 |
| Size | 20 | 20 | 195 |
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