ssr_primer
Primer Details for TRINITY_DN78866_c5_g1_i6 |
|---|
| (T)38: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGATTTTGCGCGCGAGATT | TGGTGGTGTTGGTTGGGAAA | Start: 104 |
| Tm | 59.973 | 59.959 | End: 383 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | TTGCCACGCCATTGTGATTG | GGTAGTGTGAGCACCCAGAC | Start: 491 |
| Tm | 60.038 | 60.037 | End: 746 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | TTCCCAACCAACACCACCAA | CAATCACAATGGCGTGGCAA | Start: 365 |
| Tm | 59.959 | 60.038 | End: 510 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | TTTCCCAACCAACACCACCA | CAATCACAATGGCGTGGCAA | Start: 364 |
| Tm | 59.959 | 60.038 | End: 510 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GTCTGGGTGCTCACACTACC | TCCACCATTGCCGAGATAGC | Start: 727 |
| Tm | 60.037 | 59.893 | End: 955 |
| Size | 20 | 20 | 229 |
(T)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGATTTTGCGCGCGAGATT | TGGTGGTGTTGGTTGGGAAA | Start: 104 |
| Tm | 59.973 | 59.959 | End: 383 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | TTGCCACGCCATTGTGATTG | GGTAGTGTGAGCACCCAGAC | Start: 491 |
| Tm | 60.038 | 60.037 | End: 746 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | TTCCCAACCAACACCACCAA | CAATCACAATGGCGTGGCAA | Start: 365 |
| Tm | 59.959 | 60.038 | End: 510 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | TTTCCCAACCAACACCACCA | CAATCACAATGGCGTGGCAA | Start: 364 |
| Tm | 59.959 | 60.038 | End: 510 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GTCTGGGTGCTCACACTACC | TCCACCATTGCCGAGATAGC | Start: 727 |
| Tm | 60.037 | 59.893 | End: 955 |
| Size | 20 | 20 | 229 |
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