ssr_primer
Primer Details for TRINITY_DN78847_c0_g1_i2 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAACATTCACACGCACTGT | TTCTGCAACTGCTGGATCGT | Start: 1178 |
| Tm | 59.971 | 59.965 | End: 1378 |
| Size | 20 | 20 | 201 |
| Primer 2 details |
|---|
| Sequence | ACGATCCAGCAGTTGCAGAA | ATTTGACCTCCACTGGCTCG | Start: 1359 |
| Tm | 59.965 | 60.036 | End: 1610 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | ATGAAAAACCCCACGCATGC | TTCTGCAACTGCTGGATCGT | Start: 1230 |
| Tm | 60.037 | 59.965 | End: 1378 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | CGCAGTACACTTCCACCAGT | AGACCTCGTCATAGGGGCAT | Start: 3017 |
| Tm | 59.966 | 60.105 | End: 3224 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | AGGACCCGACGATGATGGTA | TGAGCAAGCAGTACAAGCGA | Start: 319 |
| Tm | 60.106 | 59.967 | End: 457 |
| Size | 20 | 20 | 139 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAACATTCACACGCACTGT | TTCTGCAACTGCTGGATCGT | Start: 1178 |
| Tm | 59.971 | 59.965 | End: 1378 |
| Size | 20 | 20 | 201 |
| Primer 2 details |
|---|
| Sequence | ACGATCCAGCAGTTGCAGAA | ATTTGACCTCCACTGGCTCG | Start: 1359 |
| Tm | 59.965 | 60.036 | End: 1610 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | ATGAAAAACCCCACGCATGC | TTCTGCAACTGCTGGATCGT | Start: 1230 |
| Tm | 60.037 | 59.965 | End: 1378 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | CGCAGTACACTTCCACCAGT | AGACCTCGTCATAGGGGCAT | Start: 3017 |
| Tm | 59.966 | 60.105 | End: 3224 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | AGGACCCGACGATGATGGTA | TGAGCAAGCAGTACAAGCGA | Start: 319 |
| Tm | 60.106 | 59.967 | End: 457 |
| Size | 20 | 20 | 139 |
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