ssr_primer
Primer Details for TRINITY_DN78830_c1_g1_i1 |
|---|
| (T)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGGAACATCAGCAAGCCT | GGTTGGAGGGTTCATGAGGG | Start: 746 |
| Tm | 60.035 | 60.034 | End: 894 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | GAGGGAACATCAGCAAGCCT | ACTTCATGCCGAGGTTGGAG | Start: 746 |
| Tm | 60.035 | 60.036 | End: 906 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
|---|
| Sequence | GTAAACCAGGGTCGCCAAGA | GTGACACCCTGGTTCCACAA | Start: 1614 |
| Tm | 59.964 | 60.107 | End: 1881 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | TTGTGGAACCAGGGTGTCAC | CACCCCTTACAGCCTGTGAG | Start: 1862 |
| Tm | 60.107 | 60.036 | End: 1970 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | AGTAAACCAGGGTCGCCAAG | GTGACACCCTGGTTCCACAA | Start: 1613 |
| Tm | 59.964 | 60.107 | End: 1881 |
| Size | 20 | 20 | 269 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGGAACATCAGCAAGCCT | GGTTGGAGGGTTCATGAGGG | Start: 746 |
| Tm | 60.035 | 60.034 | End: 894 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | GAGGGAACATCAGCAAGCCT | ACTTCATGCCGAGGTTGGAG | Start: 746 |
| Tm | 60.035 | 60.036 | End: 906 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
|---|
| Sequence | GTAAACCAGGGTCGCCAAGA | GTGACACCCTGGTTCCACAA | Start: 1614 |
| Tm | 59.964 | 60.107 | End: 1881 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | TTGTGGAACCAGGGTGTCAC | CACCCCTTACAGCCTGTGAG | Start: 1862 |
| Tm | 60.107 | 60.036 | End: 1970 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | AGTAAACCAGGGTCGCCAAG | GTGACACCCTGGTTCCACAA | Start: 1613 |
| Tm | 59.964 | 60.107 | End: 1881 |
| Size | 20 | 20 | 269 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGGAACATCAGCAAGCCT | GGTTGGAGGGTTCATGAGGG | Start: 746 |
| Tm | 60.035 | 60.034 | End: 894 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | GAGGGAACATCAGCAAGCCT | ACTTCATGCCGAGGTTGGAG | Start: 746 |
| Tm | 60.035 | 60.036 | End: 906 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
|---|
| Sequence | GTAAACCAGGGTCGCCAAGA | GTGACACCCTGGTTCCACAA | Start: 1614 |
| Tm | 59.964 | 60.107 | End: 1881 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | TTGTGGAACCAGGGTGTCAC | CACCCCTTACAGCCTGTGAG | Start: 1862 |
| Tm | 60.107 | 60.036 | End: 1970 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | AGTAAACCAGGGTCGCCAAG | GTGACACCCTGGTTCCACAA | Start: 1613 |
| Tm | 59.964 | 60.107 | End: 1881 |
| Size | 20 | 20 | 269 |
(T)35: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGGAACATCAGCAAGCCT | GGTTGGAGGGTTCATGAGGG | Start: 746 |
| Tm | 60.035 | 60.034 | End: 894 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | GAGGGAACATCAGCAAGCCT | ACTTCATGCCGAGGTTGGAG | Start: 746 |
| Tm | 60.035 | 60.036 | End: 906 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
|---|
| Sequence | GTAAACCAGGGTCGCCAAGA | GTGACACCCTGGTTCCACAA | Start: 1614 |
| Tm | 59.964 | 60.107 | End: 1881 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
|---|
| Sequence | TTGTGGAACCAGGGTGTCAC | CACCCCTTACAGCCTGTGAG | Start: 1862 |
| Tm | 60.107 | 60.036 | End: 1970 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | AGTAAACCAGGGTCGCCAAG | GTGACACCCTGGTTCCACAA | Start: 1613 |
| Tm | 59.964 | 60.107 | End: 1881 |
| Size | 20 | 20 | 269 |
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