Primer Details for TRINITY_DN78822_c3_g2_i3 | |||
|---|---|---|---|
| (TAC)4(TA | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAAATGCTGTTGAAGGCC | CTCGGGTGAAGCGAGATCTC | Start: 1684 |
| Tm | 59.966 | 59.97 | End: 1881 |
| Size | 20 | 20 | 198 |
| Primer 2 details | |||
| Sequence | GCCAGTTGACGGTGACAGTA | ACAAAGTTGAGTTGCCCCCA | Start: 470 |
| Tm | 59.967 | 60.033 | End: 646 |
| Size | 20 | 20 | 177 |
| Primer 3 details | |||
| Sequence | ACCGGACACGCCATAAAACT | CTCGGGTGAAGCGAGATCTC | Start: 1723 |
| Tm | 59.964 | 59.97 | End: 1881 |
| Size | 20 | 20 | 159 |
| Primer 4 details | |||
| Sequence | GCCAGTTGACGGTGACAGTA | TCCCGAGCAATCTGTTTCCC | Start: 470 |
| Tm | 59.967 | 60.035 | End: 723 |
| Size | 20 | 20 | 254 |
| Primer 5 details | |||
| Sequence | TGGGGGCAACTCAACTTTGT | AAGCGATTGGTACTGCGGAA | Start: 627 |
| Tm | 60.033 | 60.037 | End: 773 |
| Size | 20 | 20 | 147 |
