ssr_primer
Primer Details for TRINITY_DN78812_c1_g1_i1 |
|---|
| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAGTTCAGACGTGTGCTC | TTTTTGCATTCCGGGCACAG | Start: 0 |
| Tm | 59.968 | 59.967 | End: 145 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
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| Sequence | TGGAGTTCAGACGTGTGCTC | TTCCGGGCACAGAAGATGAC | Start: 0 |
| Tm | 59.968 | 60.036 | End: 137 |
| Size | 20 | 20 | 138 |
| Primer 3 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | AGTATCACTTTGCGCCGACA | Start: 0 |
| Tm | 59.968 | 60.038 | End: 233 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | CTGTGCCCGGAATGCAAAAA | AGTATCACTTTGCGCCGACA | Start: 126 |
| Tm | 59.967 | 60.038 | End: 233 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | GTCATCTTCTGTGCCCGGAA | AGTATCACTTTGCGCCGACA | Start: 118 |
| Tm | 60.036 | 60.038 | End: 233 |
| Size | 20 | 20 | 116 |
(T)31: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAGTTCAGACGTGTGCTC | TTTTTGCATTCCGGGCACAG | Start: 0 |
| Tm | 59.968 | 59.967 | End: 145 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | TTCCGGGCACAGAAGATGAC | Start: 0 |
| Tm | 59.968 | 60.036 | End: 137 |
| Size | 20 | 20 | 138 |
| Primer 3 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | AGTATCACTTTGCGCCGACA | Start: 0 |
| Tm | 59.968 | 60.038 | End: 233 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | CTGTGCCCGGAATGCAAAAA | AGTATCACTTTGCGCCGACA | Start: 126 |
| Tm | 59.967 | 60.038 | End: 233 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | GTCATCTTCTGTGCCCGGAA | AGTATCACTTTGCGCCGACA | Start: 118 |
| Tm | 60.036 | 60.038 | End: 233 |
| Size | 20 | 20 | 116 |
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