ssr_primer
Primer Details for TRINITY_DN78799_c2_g5_i1 |
|---|
| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGATTCAACCATGCCCAGA | CATGTGGACAGGCATCTGGT | Start: 1135 |
| Tm | 60.033 | 60.035 | End: 1350 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
|---|
| Sequence | GCTGTCAACCTGGCAACTTG | CATGTTGCCTCCGAGTGACT | Start: 219 |
| Tm | 59.969 | 60.037 | End: 382 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | GCTGTCAACCTGGCAACTTG | TCATGTTGCCTCCGAGTGAC | Start: 219 |
| Tm | 59.969 | 60.037 | End: 383 |
| Size | 20 | 20 | 165 |
| Primer 4 details |
|---|
| Sequence | TGCTCGCCGAAAACTTCTCT | TTTTCGCTCTGCTCCAGGAG | Start: 2730 |
| Tm | 59.966 | 60.037 | End: 2829 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TGCTCGCCGAAAACTTCTCT | CTTTTCGCTCTGCTCCAGGA | Start: 2730 |
| Tm | 59.966 | 60.037 | End: 2830 |
| Size | 20 | 20 | 101 |
(ATTT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGATTCAACCATGCCCAGA | CATGTGGACAGGCATCTGGT | Start: 1135 |
| Tm | 60.033 | 60.035 | End: 1350 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
|---|
| Sequence | GCTGTCAACCTGGCAACTTG | CATGTTGCCTCCGAGTGACT | Start: 219 |
| Tm | 59.969 | 60.037 | End: 382 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | GCTGTCAACCTGGCAACTTG | TCATGTTGCCTCCGAGTGAC | Start: 219 |
| Tm | 59.969 | 60.037 | End: 383 |
| Size | 20 | 20 | 165 |
| Primer 4 details |
|---|
| Sequence | TGCTCGCCGAAAACTTCTCT | TTTTCGCTCTGCTCCAGGAG | Start: 2730 |
| Tm | 59.966 | 60.037 | End: 2829 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TGCTCGCCGAAAACTTCTCT | CTTTTCGCTCTGCTCCAGGA | Start: 2730 |
| Tm | 59.966 | 60.037 | End: 2830 |
| Size | 20 | 20 | 101 |
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