Primer Details for TRINITY_DN78723_c1_g1_i1 |
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| (A)29: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTGGATTCACTAAAAATCGATTGT | TAGCATCTACCGCTTCGCAG | Start: 75 |
| Tm | 59.851 | 59.969 | End: 175 |
| Size | 26 | 20 | 101 |
| Primer 2 details |
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| Sequence | AGTGGATTTATACGTGGATTCACT | TAGCATCTACCGCTTCGCAG | Start: 64 |
| Tm | 57.765 | 59.969 | End: 175 |
| Size | 24 | 20 | 112 |
| Primer 3 details |
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| Sequence | CGTGGATTCACTAAAAATCGATTGT | TAGCATCTACCGCTTCGCAG | Start: 76 |
| Tm | 58.457 | 59.969 | End: 175 |
| Size | 25 | 20 | 100 |
| Primer 4 details |
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| Sequence | ACGTGGATTCACTAAAAATCGATTG | TAGCATCTACCGCTTCGCAG | Start: 75 |
| Tm | 58.457 | 59.969 | End: 175 |
| Size | 25 | 20 | 101 |
| Primer 5 details |
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| Sequence | ACGTGGATTCACTAAAAATCGATTGT | TTAGCATCTACCGCTTCGCA | Start: 75 |
| Tm | 59.851 | 59.542 | End: 176 |
| Size | 26 | 20 | 102 |
