ssr_primer
Primer Details for TRINITY_DN78674_c2_g4_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTGGCTATGTCGGCACAA | TGCAGGGTGGCAAAATCTCA | Start: 9 |
| Tm | 59.964 | 60.179 | End: 135 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | GCATCCTGTCTGACAGCTCA | CGTATAACGGACGAGGGCAA | Start: 450 |
| Tm | 59.751 | 59.899 | End: 699 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
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| Sequence | GCATCCTGTCTGACAGCTCA | TTGCGTATAACGGACGAGGG | Start: 450 |
| Tm | 59.751 | 59.899 | End: 702 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
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| Sequence | AAGGACAAGGCTGCTTCTCG | CGTATAACGGACGAGGGCAA | Start: 493 |
| Tm | 60.321 | 59.899 | End: 699 |
| Size | 20 | 20 | 207 |
| Primer 5 details |
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| Sequence | AAGGACAAGGCTGCTTCTCG | TTGCGTATAACGGACGAGGG | Start: 493 |
| Tm | 60.321 | 59.899 | End: 702 |
| Size | 20 | 20 | 210 |
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