ssr_primer
Primer Details for TRINITY_DN78657_c4_g1_i1 |
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| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACTCTCGCGTCTCCACTT | CGTGTGAACCTGGTGTACCA | Start: 465 |
| Tm | 60.039 | 59.895 | End: 565 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
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| Sequence | TGGTACACCAGGTTCACACG | GGATGGGAAAGGGGAAGACG | Start: 546 |
| Tm | 59.895 | 60.107 | End: 801 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
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| Sequence | ATTCCAACTCTCGCGTCTCC | CGTGTGAACCTGGTGTACCA | Start: 461 |
| Tm | 59.826 | 59.895 | End: 565 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
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| Sequence | TGGTACACCAGGTTCACACG | TGGGATGGGAAAGGGGAAGA | Start: 546 |
| Tm | 59.895 | 60.179 | End: 803 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
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| Sequence | CAAGGACACCATTTGGCAGC | TCAACCACCAACGTCCATCC | Start: 39 |
| Tm | 60.038 | 60.251 | End: 317 |
| Size | 20 | 20 | 279 |
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