ssr_primer
Primer Details for TRINITY_DN78593_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCGTTTCGACAACACACC | CGTCGCAAAGTTGACACGTT | Start: 1942 |
| Tm | 60.042 | 59.974 | End: 2190 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
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| Sequence | ACCGGCGAAAAAGAGGGATT | GGTGTGTTGTCGAAACGAGC | Start: 1702 |
| Tm | 59.962 | 60.042 | End: 1961 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
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| Sequence | AGGCACGCCATTGGTGATAA | TCCCCGCGATGCAATTTAGT | Start: 1645 |
| Tm | 60.035 | 60.107 | End: 1834 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
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| Sequence | ACCGGCGAAAAAGAGGGATT | TCCCCGCGATGCAATTTAGT | Start: 1702 |
| Tm | 59.962 | 60.107 | End: 1834 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
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| Sequence | ACTAAATTGCATCGCGGGGA | GGTGTGTTGTCGAAACGAGC | Start: 1815 |
| Tm | 60.107 | 60.042 | End: 1961 |
| Size | 20 | 20 | 147 |
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