ssr_primer
Primer Details for TRINITY_DN78550_c0_g1_i5 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGGGAATGGCTCCTAGCT | AAGTGGGTCCGCTTTGGTAG | Start: 361 |
| Tm | 60.031 | 59.964 | End: 559 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
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| Sequence | ATAGACTGGGCCACTGGTCT | CTAATTGTCGAGCGGCAAGC | Start: 837 |
| Tm | 59.957 | 59.973 | End: 1017 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
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| Sequence | CATCCGACCATTGCATGCTG | GCCGAAAAGGTGCTGACAAG | Start: 187 |
| Tm | 59.97 | 60.04 | End: 300 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
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| Sequence | CTACCAAAGCGGACCCACTT | GATTTGTGGGAGAGGGCCAA | Start: 540 |
| Tm | 59.964 | 59.96 | End: 732 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
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| Sequence | CTTGTCAGCACCTTTTCGGC | AAGTGGGTCCGCTTTGGTAG | Start: 281 |
| Tm | 60.04 | 59.964 | End: 559 |
| Size | 20 | 20 | 279 |
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